Tag: Biotechnology

Screening of miRNAs in plasma as a diagnostic biomarker for cardiac disease based on optimization of extraction and qRT-PCR condition assay through amplification efficiency – BMC Biotechnology

Optimization of PCR efficiency PCR efficiency was first evaluated with respect to the annealing temperature using miRNA-16 and cel-miRNA-39 as endogenous and exogenous miRNA, respectively. The amplification efficiency for the…

Galacto-oligosaccharides improve barrier function and relieve colonic inflammation via modulating mucosa-associated microbiota composition in lipopolysaccharides-challenged piglets – Journal of Animal Science and Biotechnology

Experimental design The experimental protocol was approved by the Animal Care and Use Committee of Nanjing Agricultural University, and all animal care procedures were performed in accordance with Chinese Guidelines…

Maternal supply of cysteamine alleviates oxidative stress and enhances angiogenesis in porcine placenta – Journal of Animal Science and Biotechnology

Animals and experimental design This study was conducted in Guangzhou DaBeiNong Agri-animal Huabandry Science and Technology Co., Ltd. A total of 84 Landrace × York sows (parities 2–5) were allocated…